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TEAM PHILIP, with Elliott, Sierra, and Aaron
LAB 2 WRITE-UP
Descriptive Statistics
Experiment 1--Rat Study
In order to study the effects of inflammation in various dosages of LPS, a dosage of 0mg and a dosage of 10mg were given to 5 rats each. The average resulting inflammation for the 0mg dosage was 10.5 pg/ml with a standard deviation of 2.23 pg/ml and a standard error of 0.995 pg/ml. The average inflammation for the 10mg dosage was found to be 11.1 pg/ml with a standard deviation of 7.40 pg/ml and a standard error of 3.31 pg/ml.
Experiment 2--Human Study
A similar study was performed using humans with an average age of 67.7 years with a range between 60 and 82 years of age. The range between dosages was reduced in order to have more precise information. The dosages tested were 0mg, 5mg, 10mg and 15mg. For the 0mg dosage, the average inflammation was 3.83 pg/ml with a standard deviation of 1.52 pg/ml and a standard error of 0.482 pg/ml. The average inflammation for the 5mg dosage was shown to be 8.93 pg/ml with a standard deviation of 1.59 pg/ml and a standard error of 0.504 pg/ml. The average for the 10mg dosage was calculated to be 61.6 pg/ml with a standard deviation of 30.1 pg/ml and a standard error of 9.52 pg/ml. The 15mg average inflammation was found to be 658 pg/ml with a standard deviation of 213 pg/ml and a standard error of 67.3 pg/ml.
Results
Experiment 1 - Rat Study
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Experiment 2 - Human Study
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Analysis
Experiment 1 -- Human Data
ANOVA Test for Human Data:
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Bonferroni Correction (0mg vs 5mg):
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Bonferroni Correction (0mg vs 10mg):
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Bonferroni Correction (0mg vs 15mg):
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Bonferroni Correction (5mg vs 10mg):
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Bonferroni Correction (5mg vs 15mg):
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Bonferroni Correction (10mg vs 15mg):
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Experiment 2 -- Rat Data
T-Test for Rat Data:
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Summary/Discussion
In the human experiment, an ANOVA test was used and it was found that the p-value (1.4e^-16) was less than the alpha (.05). A Bonferroni Correction was also used and all of the p-values resulting from this test were less than the alpha (0.0167). With this in mind, it can be said that the results of the human test are statistically significant. As the dosage of LPS increased the amount of inflammation appeared to increase in human subjects. The results in this experiment may not represent the true results of the population because all the subjects are from one retirement home and these subjects may not represent the diversity of the entire population due to a convenience bias. In the rat experiment a t-test was used and it was found that the p-value (.888) was greater than the alpha (.05). Since the p-value is greater than the alpha, the data is not statistically significant and the inflammation in rats does not necessarily increase as the dose of LPS increases. These results likely do not represent the results of the population of rats because the sample size of rats (5 rats per group) is very small and the number of treatment groups (two) was also small.
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BME 100 Fall 2013
