BME100 f2013:W900 Group15 L1

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Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3
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OUR TEAM

Name: Justin Dombrowski
Role(s)
Name: Saiswathi Javangula
Role(s)
Name: abdulrahman alruwaythi
Role(s)
Name: Ryan Fisher
Role(s)
Name: Gage Bebak
Role(s)
Name: student
Role(s)

LAB 1 WRITE-UP

Independent and Dependent Variables

The amount of lipopolysaccharide(LPS) given is the independent variable and the amount of inflammotin produced is the dependent variable.




Experimental Design

Groups
Four groups with groups set at 0 mg, 3 mg, 6 mg, and 9 mg doses.


Eight subjects per group


Group 1 is our control with zero dose, Group 2 receives 3mg of the lipopolysaccharide, Group 3 receives 6mg of lipopolysaccharide, Group 4 receives 9mg of lipolpolysaccharide.

To test protein levels, blood samples will be drawn using ELISA.

Each person will be given their dose once a day for a month.



Subject Selection

For this experiment, 32 elderly individuals will be selected from nursing homes [8], 55+ communities [8], country clubs [8], and grocery stores [8]. They will be randomized into 4 groups of 8 making sure that there is at least 2 elderly from each location are in a group.





Sources of Error and Bias

Previous medications; Different ages; Gender; Medical History; Body weight; Amount of exercise.

We are drawing our subjects from 4 communities and dividing them up so that we have 2 subjects (1 male and 1 female) from each community in each subject group, this will account for the 8 subject members to each group. The subject age will be between 60-65. The randomness of the selection of test subjects will help to account for the varieties of medications, conditions, body statures, and exercise.

  • due to monetary constraint we are unable to aquire the amount of subjects that we were hoping to.