BME100 f2013:W900 Group14 L2

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Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3
Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6
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OUR TEAM

Name: Rachael Brard
Student
Name: Max Noble
Student
Name: Omar Eltohamy
Student
Name: Austin Davis
Student
Name: Andrew Cable
Student

LAB 2 WRITE-UP

Descriptive Statistics

Experiment 1
Human ANOVA chart.jpg
In the human experiment, the average levels of Inflammotin measured in patients who were given 0mg, 5mg, 10mg, and 15mg of the inflammatory compound Lipopolysaccharide(LPS), were 3.834 pg/mL, 8.932 pg/mL, 61.622 pg/mL, and 657.941 pg/mL, respectively. Similarly, the standard deviation for these tests were 1.52301, 1.59393, 30.11069, 212.94297, for 0mg, 5mg, 10mg, and 15mg of LPS, respectively. The standard error of the tests was .48161, .50404, 9.52183, 67.33848 for each of the tests, respectively. The median of the tests for 0mg, 5mg, 10mg, and 15mg, were 4.12 pg/mL, 8.98 pg/mL, 73.395 pg/mL, and 722.505 pg/mL, respectively. There was no mode in the data.

Experiment 2

P-value calculated by T-test for rats result: 0.8674035
The average amount of Inflammotin measured in rats for trials using 0mg and 10mg of LPS were 10.516 pg/mL, and 11.112 pg/mL, respectively. The standard deviation of the data was 2.22555, and 7.40298, for 0mg and 10mg of LPS, respectively; standard error of the data was .99529 and 3.31067 for 0mg and 10mg of LPS, respectively. The median of the data for the 0mg trial was 9.24, while for the 10mg trial, it was 8.99. There was no mode in the data.




Results

Experiment 1
Lab 2 Data.jpg
Human Data Table.jpg

  • All values are significant

Experiment 2
Lab 2 Rat Data.jpg
Rat Data Table.jpg

  • All values are significant





Analysis

Experiment 1
Due to the P-value of 1.401x10^-16 being less than the alpha level 0.05, these results show that there is a statistically significant correlation between LPS dosage and Inflammotin levels. The experiment therefore was successful in showing that an increase in LPS increased the amount of Inflammotin produced.

Experiment 2
Due to the P-value of 0.8674035 exceeding the alpha level 0.05, there is no statistic evidence to show a correlation between LPS dosage and Inflammotin levels in rats.






Summary/Discussion

It was observed that there is a statistically significant correlation between LPS dosage and Inflammotin levels in humans, but not in rats. However, only 10 rats were tested at 0mg and 10mg. Also, Inflammotin levels were somewhat negligible for humans until they received a dosage of 15mg. It is possible that with an increased dosage of LPS for the rats, more significant levels of Inflammotin would be produced. From the data and results, we can conclude that LPS at a relatively high dosage is effective for inducing Inflammotin levels in humans, while further testing may be required on rats to produce a similar correlation.