BME100 f2013:W900 Group11 L1
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LAB 1 WRITE-UP
Independent and Dependent Variables
The independent variable is the dosage of the inflammation inducing agent. The dependent variable is the the amount of inflammatory protein, Inflammotin, that appears as a result of the inflammation inducing agent.
A total of four groups will be tested, each group given a different dose of lipopolysaccharide. One group will be administered a dose of 10 mg, as this is the current established minimum dosage for demonstrating a desirable increase in inflammotin. The other groups will be administered dosages of 5mg, 1 mg, 100ug, and 0 g (placebo) of lipopolysaccharide.
Number of subjects per group
Each group will be composed of 20 people.
Concentration of inflammotin in the blood stream will be measured by an ELISA test. Twenty-five-milliliter blood samples will be drawn from the subjects at 30 min intervals after administration of the lipopolysaccharide or placebo. Blood samples will then be put through the ELISA test, and inflammotin concentration will be measured in parts per million.
All subjects have carefully been selected to create many diverse test groups. The subjects were selected based on age, but were randomized so that they represent the varied array of elderly people. Our subjects have been selected through various forms of advertisement. Subject applicants will be rejected if they are on or have been on a blood-thinner regimen.
Sources of Error and Bias
Variations in subjects can cause different reactions to the inflamatory inducing agent because of geneological differences, as well as differences in body composition. The subjects may already have medical conditions that may complicate the experiment. Failure to control certain factors like the medications that the subjects may already be taking or lifestyle would hinder the progress of the experiment. Blood work would be needed so samples will be need to be kept in contaminant-free biohazard refrigerator before testing.