BME100 f2013:W1200 Group4 L1

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Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3
Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6
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Madison Cochran
Pete Akerele-Ale
Hannah Switzer
Joshua Sarbolandi
Lindsey Macias


Independent and Dependent Variables

The independent variable is the amount of lipopolysaccharide given to the subjects. The dependent variable is the amount of Inflammotin in each subject's bloodstream.

Experimental Design

This experiment involves five test groups, each taking a different dose of lipopolysaccharide. There will be four groups taking 2 mg, 4 mg, 6mg, or 8mg of lipopolysaccharide in addition to one control group.

Number of subjects per group
Each group consists of eight subjects.

Experiment Details
The level of inflammotin in each subject will be measured in blood samples using ELISA at the beginning of the test and every seven days following the commencement of the test for the next three months. Each subject will be assigned a set of pills at the beging of the test based on the group they have been placed in. The subjects will take their assigned dose once daily at 12 p.m. for the duration of the three month testing period.

Subject Selection

Each group of eight subjects will consist of four males and four females. Within each group there will be both a male and a female in each of the following age ranges: 60-64, 65-69, 70-74, and 75-79. These subjects will be randomly selected in order to represent a variety of healths, life-styles, locations, and ethnic backgrounds.

Sources of Error and Bias

One source of bias occurred when we chose our test subjects. Our subjects' ages ranged from sixty to eighty years and left out any ages above the national average age. One possible way to control this source would be to expand the number of subjects per group to include ages ranging form eighty to one hundred years. A source of error occurred between the measurement per pill of lipopolysaccharide. Our test amounts only include even numbers bellow ten. To control this error, we could add more groups to add various other measurement between the chosen milligrams per pill. Another possible source of error could have occurred when choosing test subjects. If any of our subjects had a preexisting health condition and/or were taking any medication, these unknown variables could have an effect on their body's ability to produce Inflammotin. In order to control this source of error, we would have to screen any subjects with preexisting health conditions as well as know of any medication the subjects had or are taking.