BME100 f2013:W1200 Group2 L1

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Name: Amber Bengson
Name: Chandler Varone
Name: Emily Mei
Name: Terri Bivins
Name: Prycilla Jones
Name: student


Independent and Dependent Variables

The independent variable in this experiment is the dosage of lipopolysaccharide. The dependent variable is the presence of Inflammotin in the bloodstream.

Experimental Design


Dosage Levels: 0 mg, 2 mg, 4 mg, 6 mg, 8 mg
(65-69 y/o, 70-74 y/o, 75-79 y/o, 80-84y/o)

No serious health issues
Not taking any medications that would interfere with the administering of Inflammotin
Dietary guidelines--no high salt or dairy intake

Number of subjects per group

400 people total in the test sample
200 men, 200 women
Groups of 100 within each age range from 65-85 years old (increments of 5 years)
20 people in each age bracket will be administered one of the following dosage amounts: 0 mg, 2 mg, 4 mg, 6 mg, 8 mg
Within each dosage amount in each age bracket, there will be 10 women and 10 men tested.


The groups will be administered dosages (0 mg, 2 mg, 4 mg, 6 mg, 8 mg) on a specific date and time, once a week for a period of four months. ELISA tests will be run before administering the next dose each week.

Subject Selection

-Subjects are to be at a minimum age of 65 and a maximum age of 84.
-Subjects should not be taking any specific medication or drugs that influences their level of protein.
-Subjects cannot be diagnosed with any sort of inflammatory conditions or serious health issues.
-Equal amount of male and female subjects will be selected.
-For each age group, there will also be an equal amount of male and female subjects.

Sources of Error and Bias

(What are some of the potential sources of error or biases that could affect your results and how would you control for them?)

-The subject not disclosing prior medications/supplements each took prior and during the experiment.

-Whether or not the subject actually took the pill.

-Diets that are high in sodium or dairy can increase inflammation. If a subject has a diet high in either sodium or dairy it can affect the results of the experiment.

-Have the subject document what pill/medication they took before and during the experiment, what dosage, and what time they took it.