BME100 f2013:W1200 Group1 L1
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LAB 1 WRITE-UP
Independent and Dependent Variables
Dependent variable: the amount of inflammatory protein (Inflammotin) in the blood samples of test subjects
Number of subjects per group
Each group of 100 people will be given a different dosage of LPS through a pill. Group 1 will be the control group and will not take any LPS. Each group will take more LPS in increments of 3 mg, ranging from doses of 0mg-30mg. The subjects will take one dose per day for three months. At the end of every week, the test subjects' blood will be tested using ELISA for increased/decreased inflammation protein levels. The data from the test subjects in each of the eleven groups will be averaged and compared at the end of the three months.
The goal by the end of the experiment is to determine the lowest dosage of lipopolysaccharide (LPS) in order to increase the inflammatory protein (Inflammotin) in the elderly.
Sources of Error and Bias
The experimental budget is limited, which affects the sample size and selection and can cause inaccurate results. The test subjects will not be screened for pre-existing health conditions, which may affect the overall response to the medication. The test subjects may be on different types of medication which could cause a counter effect on the LPS. In order to better control this, test subjects will be asked to limit the number of additional drugs and medications throughout the experiment. The experimental design is not a double-blind procedure. This may cause the distributor and the test subjects to be biased on the decisions of whether the LPS is effective or not. The control group should be given a sugar pill so that they will not know if they are being dossed or not. The location, ethnicity, and race of the test subjects can affect the response to the LPS as well. <------Mario and Nima contributed and helped Jacqueline