BME100 f2013:W1200 Group17 L1

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Independent and Dependent Variables

(Identify the independent and dependent variable in the mini-problem.)

The independent variable is the amount of lipopolysaccharide administered to the patient, measured in milligrams.

The dependent variable is the amount of inflammotin found in the patient's blood sample, measured in milligrams per milliliter.

Control Groups

Positive Control Group: Subjects receiving 12 milligrams of lipopolysaccharide.

Negative Control Group: Subjects receiving 0 milligrams of lipopolysaccharide.

The subjects receiving 0 mg of lipoplysaccharide are the negative control group because they are not receiving any milligrams of lipopllysacchride, therefore there will not be any increase of protein during inflammation.

The subjects receiving 12 mg of lipoplysaccharide are the positive control group because we know that 10 mg of lipopollysacchride will increase protein during inflammation in the elders.

Experimental Design


Number of subjects per group

(Add details about your experimental design)

Subject Selection

(Discuss considerations of how subjects are to be selected.)
7 people per group ( Age Groups ) 1) 45-50 years of age 2) 50-55 years of age 3) 55-60 years of age 4) 60-65 years of age 5) 65-70 years of age 6) 75-80 years of age 7) 80-85 years of age Contributed by: Kevin Luong

Sources of Error and Bias

(What are some of the potential sources of error or biases that could affect your results and how would you control for them?)

Blood type is one source of error in this experiment. DIfferent blood types may contain different levels of inflammatory proteins that could affect the data. The amount of protein consumed by a person could possibly affect the number of proteins in the blood simply because of consumption. A lifestyle of bad habits such as drinking and smoking can affect the levels of inflammatory proteins due to the negative effects alcohol and drugs can have on the body. Weight class will be a bias because subjects should be similar weights since the effect of the drug could be weaker for a larger person versus a smaller person.