BME100 f2013:W1200 Group16 L1

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Owwnotebook icon.png BME 100 Fall 2013 Home
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Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3
Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6
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OUR TEAM

Name: Courtney Willson
Role(s)
Name: Ayanna Akinyemi
Role(s)
Name: Rene Reynolds
Role(s)
Name: John Jakoubek
Role(s)
Name: Mychal Hooser
Role(s)

LAB 1 WRITE-UP

Independent and Dependent Variables

Independent Variable: Amount of lipopolysaccharide

Dependent Variable: Level of Inflammotin




Experimental Design

Groups
6

Number of subjects per group
10 subjects per group

Group 1- Placebo (0 mg of LPS)

Group 2- 6 mg of LPS

Group 3- 8 mg of LPS

Group 4- 10 mg of LPS

Group 5- 12 mg of LPS

Group 6- 14 mg of LPS

  • We chose above and below 10 mg of LPS because some people are more susceptible to change than others

1 dose a day for 30 days

Take blood sample twice a week for a month

Measure the amount of Inflammotin in the blood via ELISA

Compare results of groups 1 though 6. Graph the results of amount of LPS with the amount of Inflammotin in the blood

Using the graph, make conclusions about the effect of LPS on the amount of Inflammotin in the blood




Subject Selection


Age: 2 from 50's, 2 from 60's, 2 from 70's, 2 from 80's, 2 from 90's

Gender: 5 Females and 5 Males/Group

Weight/Height: 3 from below average weight, 3 from average weight, 4 from above average weight per group

Varying health conditions (some might have cancer or be a survivor, some might have heart problems, some might have down syndrome or other mental diseases)




Sources of Error and Bias


Time of day (Take medication and blood test at the same time of day) - inconsistency in results; allows for more control in data

Additional medications (Record any additional medications subjects are taking) - negate or enhance the effect of the drug