BME100 f2013:W1200 Group14 L1

BME 100 Fall 2013	Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Name: student Role(s) Name: student Role(s) Name: student Role(s) Name: student Role(s) Name: student Role(s) Name: student Role(s) LAB 1 WRITE-UP Independent and Dependent Variables Independant: Amount of the LPS distributed to the "Elderly" in each group. Dependant: Inflamation in the Elderly caused by "infammotin". Experimental Design Groups Control group Group taking LPS Number of subjects per group 30 subjects per group 1) Measure initial amounts of inflammatin in the blood of all subjects before treatment. 2) Administer weekly doses of the LPS to the experimental group, starting at 10 mg and increasing the dose by 5mg a week for 10 weeks. 3) Take blood samples from all subjects weekly to analyze inflammatin levels in the blood using ELISA before the administration of the weekly dose of the LPS. 4) Repeat this experiment with different subject groups in Canada, Mexico, and the United States of America. 5) Make sure to have three trials in each country in order to establish reliability. Subject Selection The subjects are 30 males and 30 females ranging from the age of 60 to 75. To choose our subjects, those that were interested filled out an application. They would then be selected based on their medical history, age, lifestyle, allergies, medications, and diet. This experiment consists of middle class working citizens from North America. They are all in good health with no allergies to any medicine. All the subjects work strenuous jobs that will invoke inflammation. Some examples of these jobs are manual laborers, military veterans, athletes, caretakers, firefighters, police officers, etc. Sources of Error and Bias Some sources of bias could be: -Health Control:Do not allow people with terminal illnesses to participate, due to the fact that said illnesses might affect blood work. Have researchers combine a list of illnesses that would affect bloodwork or might appear to mask or heighten the presence of inflammatin, and make sure to avoid choosing subjects that have these illnesses. -Allergies Control: Make sure subjects are tested for LPS prior to the research study, and subjects that are allergic should not be allowed to participate in the study. -Lifestyle Control: When choosing the subjects and sample size, make sure to pick people who have tried to maintain active and healthy lifestyles. Also, make sure that other attributes, namely ethnicity, gender, and socioeconomic status are varied during selection. These characteristics can be found through the surveys and variety can be ensured through the researchers' choice. -Age Control: Only people of the ages of 60-75 should be allowed to participate. Some sources of error could be: -Other medication that could interfere with the study: Control: Researchers should make a list of medications that might have a chance of interfering with the function of LPS, by either heightening or masking the presence of inflammatin. Subjects should be sure to list all the medications that they are using at the time, and their bloodwork should be taken prior to the study in order to make sure that interfering medications are not being taken. Also, subjects should be given a list of medications that would affect the study and should be told to avoid taking them, or to avoid the study. -Diet Control: Researchers should make a list of foods that might affect the absorption of LPS or the presence of inflammatin, and this list should be given to all the subjects. The subjects should avoid taking these foods for the duration of the study.