BME100 f2013:W1200 Group10 L1
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LAB 1 WRITE-UP
Independent and Dependent Variables
In this experiment, the dosage of lippolysaccharides serves as the independent variable and the amount of inflammotin serves as the dependent variable.
At the beginning of the study each group will have blood drawn to determine baseline inflammation for each individual. Blood samples will be prepared appropriately and tested for Inflammotin using ELISA assays. Immediately after blood being drawn the appropriate dose of LPS will be administered to the subjects. Subjects will not know which group they are in. Subjects will then be kept in a controlled environment to minimize variance in activity and diet that might cause variance in Inflammotin concentrations. At a specific amount of time after the LPS dose has been administered the subjects will have blood drawn a second time. The amount of time between dosage and the second drawing of blood will be the same for all subjects. The samples will be prepared appropriately and tested for Inflammotin using ELISA assays. Analysis will first test for change in Inflammotin concentrations from the first sample to the second sample with respect to individual subjects. These changes in concentration will then be compared between groups using ANOVA method and Bonferroni correction to test for statistically significant increases in Inflammotin concentrations dependent on dose of LPS.
The subjects will be randomly selected and split into four groups of twelve. Six people of each gender will make up each group. The subjects will be aged sixty to seventy years. They will be of good health during the test, and will have no previous inflammatory issues or exposure to inflammatory drugs.
Sources of Error and Bias
There are many different biases and errors that could affect this type of experiment, primarily due to the experiment being conducted in the human body. Due to the subjects being of older age, sickness and any other types of failures in the body can cause error because it would skew the data of the experiment. In addition, pertaining more to the drug, some subjects may be allergic to it or cannot properly metabolize it, causing problems with the data and thus causing error. Sample contamination of the blood can also be a huge error with the experiment because it will cause a different output once the drug LPS is added in. Lifestyle of the subjects will also greatly affect the data of this experiment because a person that is healthier will have a better immune system and their inflammation reaction may be different than someone that is not as healthy as them.