BISC220/S13:Series 3
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Lab 8: Cell Culture
Lab 9: Apoptosis - DNA
Lab 10: Apoptosis - Protein 1
Lab 11: Apoptosis - Protein 2
Lab 12: Imaging Presentations
Media Recipes
Schedule of Experiments
| Lab # | Dates | Activity | Outside lab time |
|---|---|---|---|
| 8 | 4/1 - 4/5 | Introduction to Cell Culture | Cell passage and maintenance Work on Paper 2 |
| 9 | 4/9 - 4/12 | 1) Isolation of HL-60 genomic DNA 2) Agarose Gel Electrophoresis 3) Paper 2 due |
Find paper for Presentations |
| 10 | 4/16 - 4/19 | 1) Preparation of Protein Extracts 2) SDS-PAGE Gel 3) Examine fixed HL-60 cells |
Work on Presentations and Final Paper |
| 11 | 4/23 - 5/1 | PARP-1 Western Blot | Work on Presentations and Final Paper |
| 12 | 4/30 - 5/8 | Imaging Presentations | Final Paper due for everyone - Wed., May 8 |
