BISC219/F13: Media Recipes

Ingredients and Stock Concentrations for some of the reagents used in Series 3

Reagent	Composition
Luria-Bertoni Medium (LB)	1% Tryptone 0.5% Yeast Extract 1% NaCl (2% agar in plates)
Nematode Growth Medium Lite(NGM lite)- Available in powder form from US Biological Cat. #N1005 (http://www.usbio.net)	0.3% NaCl 1.7% Bacto-agar 0.25% Bacto-peptone 0.1% (vol/vol)cholesterol
Ampicillin*	Stock = 50 mg/ml in water use at 1:1000 in liquid cultures use at 1:500 in LB amp plates
Tetracycline*	Stock = 12.5 mg/ml in water use at 1:1000 in liquid cultures and in solid medium plates
IPTG	Stock = 0.5 M use 0.5 mM in cultures
1x PBST (phosphate buffered saline with Tween20)	137 mM NaCl,2.7 mM KCl,4.3 mM Na2HPO4,1.47 mM KH2PO4, 0.25% -Adjust to a final pH of 7.4.

*NOTE: These concentrations are STOCK concentrations - not effective concentrations!