BISC219/F13: Assignment Series3 Reverse Genetics using RNAi

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Assignment Results Section on Experimental Work in Series 3 Reverse Genetics Using RNAi

Please use the feedback from your Series 1 and 2 Results Sections to improve your final Results Section for our Series 3 Reverse Genetics Project. Questions that should be addressed from these experiments include the following: What is the function of hsf-1 in C. elegans? Is Did we successfully downregulate or silence hsf-1 expression with RNAi? Is the rrf-3 mutant strain of C. elegans enhanced for RNAi compared to N2 (wild type) nematodes? Is the genetically modified CL2070 strain of C. elegans a effective reporter of gene expression regulation of HSP-16.2? Note that the order in which these questions are listed is NOT necessarily the best order to address them in your Results analysis. Please include a TITLE for this paper.
This assignment is due for all students at 4pm on the last day of classes, Dec. 10.

Grading Rubric

Rubric Results Section for Series 3 BISC219 F13: Reverse Genetics Project – 27 points

At or Above Standard Below Standard Possible


Title/Structure 1.5-2 pt. Structure of the narrative presents information in a logical sequence. Refers to figures/tables by number in the narrative & inserts figures/tables into narrative appropriately. Denotes yourself as first author and your partner as second author. Title is appropriate for goals, findings and conclusions. 0-1 pts. Did not impart information in a logical sequence.Did not reference figures/tables or insert them appropriately into the narrative. Missing or inappropriate title.

Does not include authorship.

2 __/2
&/or Figure(s)
Table(s) and/or Figure(s) well designed to illustrate conclusions. Included all crucial information that allows the figure or table to make the main points visually and to “stand alone”: novice reader does not need to read the narrative or the legend to see the data’s main meaning. All data adequately identified; correct units included; labeling appropriate. Figure(s) or table(s) not well designed to illustrate main points visually, clearly, or in most direct and simple way or missing essential information needed for understanding. 10 __/10
Data Analysis/
13-15pts. Identifies question addressed; includes a brief description of the experimental design related to the question; Uses appropriate experimental evidence and explains it effectively to a general audience. Includes all parts requested in assignment description. Writing style uses correct vocabulary, grammar, spelling, punctuation, and is clear and concise. Appropriate conclusions come logically from investigation's findings 0-12.5pts. Missing question addressed, experimental design, appropriate experimental evidence or an inadequate explanation of the evidence. Does not include all aspects requested in assignment description. Inappropriate level of detail (either too much detail or too general). Writing style is not clear or concise and/or uses incorrect vocabulary, grammar, spelling, or punctuation. Unsupported conclusions or inappropriate conclusions 15 __/15
Total 27 __/27