Wittrup:Protocols: Difference between revisions
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[[Wittrup:Buffers]] | [[Wittrup:Buffers]] | ||
[[Wittrup: | [[Wittrup:Yeast media]] | ||
===Assays=== | |||
=== | ===DNA=== | ||
=== | ===RNA=== | ||
===Proteins=== | ===Proteins=== | ||
=== | [[Wittrup: Determining Protein Concentration]] | ||
[[Wittrup:His-Tag Proteins/Nickel Column Purification (suppliers, troubleshooting, etc.)]] | |||
[[Wittrup:FPLC general use]] | |||
[[Wittrup:SDS-PAGE]] | |||
[[Wittrup:ECL Western Blots and Phosphorimager]] | |||
[[Wittrup:Protein concentration by ultrafiltration]] | |||
===Yeast Protocols=== | |||
[[Wittrup: Yeast Transformation]] | |||
[[Wittrup: Plasmid rescue from yeast]] | |||
[[Wittrup: Yeast storage and revival]] | |||
[[Wittrup: Yeast surface display]] | |||
=== | ===Cell culture=== | ||
=== | ===Software=== |
Revision as of 17:35, 15 January 2006
Buffers/Equipment/Media
Assays
DNA
RNA
Proteins
Wittrup: Determining Protein Concentration
Wittrup:His-Tag Proteins/Nickel Column Purification (suppliers, troubleshooting, etc.)
Wittrup:ECL Western Blots and Phosphorimager
Wittrup:Protein concentration by ultrafiltration
Yeast Protocols
Wittrup: Plasmid rescue from yeast
Wittrup: Yeast storage and revival
Wittrup: Yeast surface display