User:Wesley J. Houston/Notebook/MAP/2013/03/22: Difference between revisions
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(Autocreate 2013/03/22 Entry for User:Wesley_J._Houston/Notebook/MAP) |
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{| class="wikitable" border="0" cellspacing="3" <!-- Digest rxn. table --> | |||
|-valign="top" | |||
| <u>Reagent</u> || <u>Volume</u> || | |||
| rowspan="9" | [[Image:Electrophoresis4.jpg |150px|Results of third electrophoresis]]<br>15 μL/lane, 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] | |||
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| DNA (plasmid) || 20 μL | |||
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| 10x buffer || 3.0 | |||
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| enzyme 1 (Pst1) || 1.0 | |||
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| enzyme 2 (Xba1 or Spe1)|| 1.0 | |||
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| dH<sub>2</sub>O || 5.0 μL | |||
|- | |||
| || 30 μL --> 37°C/ ~15 min. | |||
|} | |||
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Revision as of 15:26, 25 March 2013
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3/22/13
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