3/25/13
- Prepped plasmids of KAH201 and determined concentration and purity.
- Followed by digestion and gel electrophoresis, then purification.
- However, concentrations were determined to be too low to complete a ligation.
- More liquid cultures of KAH182 and KAH201 were prepped.
- Colonies placed into LB broth overnight in shaking 37C incubator.
| Sample |
260/280 |
ng/μL
|
| 1. KAH201 (Plasmid) |
1.795 |
54.054
|
| 2. KAH201 (Plasmid) |
1.708 |
47.244
|
| Reagent |
Volume |
|
30 μL/lane, 1% agarose; Ladder
|
| DNA (plasmid) |
25 μL
|
| 10x buffer |
3.0
|
| enzyme 1 (Pst1) |
1.0
|
| enzyme 2 (Xba1 or Spe1) |
1.0
|
| dH2O |
0.0 μL
|
| |
30 μL --> 37°C/ ~15 min.
|
> Measure conc.'s of gel purified fragments
| Sample |
260/280 |
ng/μL
|
| 1. KAH201 (S/P) |
0.727 |
2.54
|
| 2. KAH182 (X/P) |
0.789 |
1.596
|
- Due to the low concentrations, I made more liquid cultures of KAH182 and KAH201.
- Followed the established protocol for making liquid cultures: pick one colony and place into LB broth overnight in shaking 37C incubator.
|
Project name
Main project page
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