User:Tara K. Luckau/Notebook/Team ConGen/2013/02/09: Difference between revisions
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|style="background-color: #BCED91"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Tara's Lab Notebook</span> | |style="background-color: #BCED91"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Tara's Lab Notebook</span> | ||
|style="background-color: #9DB68C" align="center"| | |style="background-color: #9DB68C" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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== | ==ASHY Cvanµ23== | ||
===Gel=== | |||
{| {{table}} style="text-align: center" | |||
|- | |||
! Pour !! Load !! Run | |||
|- | |||
| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V | |||
|- | |||
| 27 µL GelRed || 6 µL ladder || 45 minutes | |||
|} | |||
[[Image:20130209_Gela.png|800 px]] | |||
* mispriming everywhere - primer pair not usable for ASHY | |||
==ASHY Cvanµ24== | |||
===PCR=== | |||
* [[Image:20130209_PCRa.png|800 px]] | |||
===Gel=== | |||
{| {{table}} style="text-align: center" | |||
|- | |||
! Pour !! Load !! Run | |||
|- | |||
| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V | |||
|- | |||
| 27 µL GelRed || 6 µL ladder || 45 minutes | |||
|} | |||
[[Image:20130209_Gelb.png|800 px]] | |||
* it works! Yay! Many possibilities | |||
==ASHY Cvanµ26== | |||
===PCR=== | |||
* [[Image:20130209_PCRb.png|800 px]] | |||
===Gel=== | |||
{| {{table}} style="text-align: center" | |||
|- | |||
! Pour !! Load !! Run | |||
|- | |||
| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V | |||
|- | |||
| 27 µL GelRed || 6 µL ladder || 45 minutes | |||
|} | |||
[[Image:20130209_Gelc.png|800 px]] | |||
* mispriming everywhere - primer pair not usable for ASHY | |||
==ASHY Cvanµ27== | |||
===PCR=== | ===PCR=== | ||
* [[Image: | * [[Image:20130209_PCRc.png|800 px]] | ||
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[[Image: | [[Image:20130209_Geld.png|800 px]] | ||
* mispriming everywhere - primer pair not usable for | * mispriming everywhere - primer pair not usable for ASHY | ||
Latest revision as of 22:27, 26 September 2017
Tara's Lab Notebook | Main project page Previous entry Next entry | ||||||||||||||||||||||||||||||||||||
ASHY Cvanµ23Gel
ASHY Cvanµ24PCR
Gel
ASHY Cvanµ26PCR
Gel
ASHY Cvanµ27PCR
Gel
|