Difference between revisions of "User:Tara K. Luckau/Notebook/Team ConGen/2013/02/08"

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(Autocreate 2013/02/08 Entry for User:Tara_K._Luckau/Notebook/Team_ConGen)
 
(fix raw html notebook nav)
 
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|style="background-color: #BCED91"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Tara's Lab Notebook</span>
 
|style="background-color: #BCED91"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Tara's Lab Notebook</span>
|style="background-color: #9DB68C" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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|style="background-color: #9DB68C" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
 
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==CNTI Acos5==
+
==ASHY Cvanµ18==
 +
 
 +
 
 +
===Gel===
 +
 
 +
{| {{table}} style="text-align: center"
 +
|-
 +
! Pour !! Load !! Run
 +
|-
 +
| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V
 +
|-
 +
| 27 µL GelRed || 6 µL ladder || 45 minutes
 +
|}
 +
 
 +
 
 +
[[Image:20130208_Gela.png|800 px]]
 +
 
 +
* mispriming everywhere - primer pair not usable for ASHY
 +
 
 +
 
 +
==ASHY Cvanµ19==
  
  
 
===PCR===
 
===PCR===
* [[Image:20120110_PCRa.png|800 px]]
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* [[Image:20130208_PCRa.png|800 px]]
  
  
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[[Image:20120110_Gela.png|800 px]]
+
[[Image:20130208_Gelb.png|800 px]]
  
* mispriming everywhere - primer pair not usable for CNHY
+
* no amplification - primer pair not usable for ASHY
* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
+
 
* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]
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 +
==ASHY Cvanµ23==
 +
 
 +
 
 +
===PCR===
 +
* [[Image:20130208_PCRb.png|800 px]]
  
  

Latest revision as of 21:27, 26 September 2017

Owwnotebook icon.png Tara's Lab Notebook Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png


ASHY Cvanµ18

Gel

Pour Load Run
270 mL 1x TAE + 5.4 g agarose 2 µL gel load dye + 4 µL PCR product 160 V
27 µL GelRed 6 µL ladder 45 minutes


20130208 Gela.png

  • mispriming everywhere - primer pair not usable for ASHY


ASHY Cvanµ19

PCR

  • 20130208 PCRa.png


Gel

Pour Load Run
270 mL 1x TAE + 5.4 g agarose 2 µL gel load dye + 4 µL PCR product 160 V
27 µL GelRed 6 µL ladder 45 minutes


20130208 Gelb.png

  • no amplification - primer pair not usable for ASHY


ASHY Cvanµ23

PCR

  • 20130208 PCRb.png