Difference between revisions of "User:Moira M. Esson/Notebook/CHEM-571/2013/10/09"

From OpenWetWare
Jump to: navigation, search
(Kinetics)
(Kinetics)
Line 25: Line 25:
 
<br>
 
<br>
 
'''UV/vis'''       
 
'''UV/vis'''       
 +
Figure 1.
 +
<br>
 +
[[Image:Adenosine Concentrations vs. Time.png]]
 +
<br>
  
 
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
 
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Revision as of 10:51, 16 October 2013

Owwnotebook icon.png Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Objectives

  1. Measure the kinetics of the conversion of adenosine to inosine using ADA as a catalyst, in the presence of the inhibitor EHNA.


Kinetics

General Protocol:

  • The general protocol followed the general protocol described on 2013/10/08.
  • A new solution of 40μM adenosine solution was prepared following the protocol described on 2013/10/08.
  • 2μL EHNA was added to the prepared adenosine solution for approximately 1 minute before placing the solution in the cuvette.
  • A more detailed protocol is described in Matt Harting's notebook


Prepared solutions:


EHNA stock 5mg EHNA in 1mL DMSO ---> 15.9mM EHNA (1.9μL)(15.9mM EHNA)=(10mL)(C2). C2 ---> 3μM EHNA The reaction samples will contain roughly 1nM EHNA
UV/vis Figure 1.
Adenosine Concentrations vs. Time.png