Difference between revisions of "User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/10/09"

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(Samples Prepared)
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==October 2, 2013==
 
 
===Procedures===
 
*Absorbance measurements and Beer's law calculations for samples from 10/25/2013
 
*Sample preparation based on calculated concentrations
 
 
===Data===
 
=====Absorbance Measurements from 10/25/2013=====
 
[[Image:2013_1002_ZnPPIX_from_last_week.PNG]]<br.>
 
Beer's Law calculations:<br.>
 
[[Image:2013_1002_ZnPPIX_from_last_week_calcs.PNG]]
 
 
=====Absorbance Measurements, Today=====
 
I decided to run two more samples at higher concentrations on the UV-vis and calculate the concentrations from these because I needed higher concentrations in order to make my samples at the concentrations I've been using.
 
<br.>
 
[[Image:2013_1002_ZnPPIX_for_calcs.PNG]]<br.>
 
Beer's Law calculations:<br.>
 
[[Image:2013_1002_ZnPPIX_calcs.PNG]]
 
 
=====Samples Prepared=====
 
"20uM" and "50uM" ZnPPIX dilutions made today, fresh aliquots of all three DNA strands, and 23.5nM AuNps were used to prepare these samples
 
*2.47uM ZnPPIX
 
*2.47uM ZnPPIX/1.11uM DNA-SH
 
*2.47uM ZnPPIX/1.11uM DNA (4)
 
*2.47uM ZnPPIX/1.11uM DNA (8)
 
*1.934uM ZnPPIX/0.94uM DNA-SH/11.75nM AuNPs
 
*1.934uM ZnPPIX/0.74uM DNA (4)/11.75nM AuNPs
 
*1.934uM ZnPPIX/0.74uM DNA (8)/11.75nM AuNPs
 
*2.47uM ZnPPIX/11.75nM AuNPs
 
 
===Notes===
 
*Run today's samples for absorbance and fluorescence
 
*Ask Dr Hartings for more ZnPPIX
 
  
 
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Revision as of 08:35, 2 October 2013

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