User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/06/10: Difference between revisions
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Use 0.0094g NaH<sub>2</sub>PO<sub>4</sub> and 1.19188g Na<sub>2</sub>HPO<sub>4</sub> in 1L water.<br.> | Use 0.0094g NaH<sub>2</sub>PO<sub>4</sub> and 1.19188g Na<sub>2</sub>HPO<sub>4</sub> in 1L water.<br.> | ||
===New: Modifying Silica-Coated AuNPs with HS-DNA Procedure=== | ===New: Modifying Silica-Coated AuNPs with HS-DNA Procedure=== | ||
from [http://pubs.acs.org/doi/full/10.1021/la7019846 Silica-Coated, Au/Ag Striped Nanowires for Bioanalysis] | adapted from [http://pubs.acs.org/doi/full/10.1021/la7019846 Silica-Coated, Au/Ag Striped Nanowires for Bioanalysis] | ||
====Reactions==== | ====Reactions==== | ||
=====Fuctionalizing the AuNPs@SiO<sub>2</sub> with NH<sub>2</sub>===== | =====Fuctionalizing the AuNPs@SiO<sub>2</sub> with NH<sub>2</sub>===== |
Revision as of 12:38, 20 June 2013
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June 10, 2013
Buffer Preparation and CalculationsSodium phosphate buffer, pH 9, to be used for the modification of HS-DNA and AuNPs@SiO2 procedure.<br.> monosodium phosphate- MW: 119.98g/mol<br.> disodium phosphate- MW: 141.96g/mol, pKA: 6.82<br.> buffer- pH 9,concentration 10mM<br.> <br.> 9=6.82+log([base]/[acid])<br.> 151.36=(10-[base])/([acid])<br.> [acid](151.36+1)=10<br.> [acid]=0.066mM<br.> [base]=9.935mM<br.> <br.> Use 0.0094g NaH2PO4 and 1.19188g Na2HPO4 in 1L water.<br.> New: Modifying Silica-Coated AuNPs with HS-DNA Procedureadapted from Silica-Coated, Au/Ag Striped Nanowires for Bioanalysis ReactionsFuctionalizing the AuNPs@SiO2 with NH2AuNPs@SiO2(aq*)+H2N(CH2)3Si(OCH3)3(l)→AuNPs@SiO2-NH2(aq*)<br.> '*'denotes colloidal system Activating the NH2 Functionalized AuNPs@SiO2 with Sulfo-SMCCAuNPs@SiO2-NH2(aq*)+C16H17N2O9SNa(aq**)→SSMCC-Activated AuNPs@SiO2-NH2(aq*)<br.> '*'denotes colloidal system<br.> '**"denotes dissolution in buffer, not water Attaching the Thiolated DNASSMCC-Activated AuNPs@SiO2-NH2(aq*)+HS-DNA(aq)→Crosslinked S-DNA and AuNPs@SiO2-NH(aq*)<br.> '*'denotes colloidal system<br.> Chemicals Needed and Safety
Procedure
Concentrations may be doubled.<br.> Calculations |