Difference between revisions of "User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2013/04/11"

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(Remains of the Day)
(Remains of the Day)
 
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* P. syringae pv. tomato isolate DC3000 has an optical density at 600 nm (OD600) of 0.1 (∼5 × 107 CFU/ml). I am simply going to adjust all of my cultures of Pst and Xg to OD=0.1, innoculate the plants, and go from there - seeing which one gives the best result. Later I can figure out a precise CFU count for a specific dilution when I settle on an isolate.  
 
* P. syringae pv. tomato isolate DC3000 has an optical density at 600 nm (OD600) of 0.1 (∼5 × 107 CFU/ml). I am simply going to adjust all of my cultures of Pst and Xg to OD=0.1, innoculate the plants, and go from there - seeing which one gives the best result. Later I can figure out a precise CFU count for a specific dilution when I settle on an isolate.  
 
* Stirred and watered soil for strawberry experiment - both fish and mustard meal treatments had fungal blooms going on.  
 
* Stirred and watered soil for strawberry experiment - both fish and mustard meal treatments had fungal blooms going on.  
 +
* Found great publication, "Survival of gfp-tagged antagonistic bacteria in the rhizosphere of tomato plants and their effects on the indigenous bacterial community" - http://onlinelibrary.wiley.com/doi/10.1111/j.1574-6941.2006.00093.x/abstract
  
 
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Remains of the Day

  • P. syringae pv. tomato isolate DC3000 has an optical density at 600 nm (OD600) of 0.1 (∼5 × 107 CFU/ml). I am simply going to adjust all of my cultures of Pst and Xg to OD=0.1, innoculate the plants, and go from there - seeing which one gives the best result. Later I can figure out a precise CFU count for a specific dilution when I settle on an isolate.
  • Stirred and watered soil for strawberry experiment - both fish and mustard meal treatments had fungal blooms going on.
  • Found great publication, "Survival of gfp-tagged antagonistic bacteria in the rhizosphere of tomato plants and their effects on the indigenous bacterial community" - http://onlinelibrary.wiley.com/doi/10.1111/j.1574-6941.2006.00093.x/abstract