User:Carly M. Montanero/Notebook/CHEM-571/2013/11/19: Difference between revisions

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==Entry title==
==Objective==
* Insert content here...
To synthesize invertase-AuNPs and BSA-AuNPs.


==Procedure==


'''Creating the Gold Stock Solution'''
# Add 0.0524 g of HAuCl<sub>4</sub>·3H<sub>2</sub>O to a 50 mL volumetric flask and fill with distilled water.
# The final concentration of gold stock was 2.66 mM.
'''Creating the Invertase Stock Solution'''
# Add 0.0717 g of lysozyme to a 50 mL volumetric flask and fill with distilled water.
# The final concentration of lysozyme stock was 100.28 μM.
'''Creating the BSA Stock Solution'''
# Add 0.0117 g of hemoglobin to a 50 mL volumetric flask and fill with distilled water.
# The final concentration of hemoglobin stock was 15 μM.
Solutions with the following Au:protein ratio were synthesized:
:''Gold to Hemoglobin Ratio''
::*30
::*50
::*70
::*90
::*110
::*130
::*150
::*170
::*190
::*210
:''Gold to Lysozyme Ratio''
::*5
::*10
::*15
::*20
::*25
::*30
::*35
::*40
::*45
::*50
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__NOTOC__
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Revision as of 10:41, 19 November 2013

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Objective

To synthesize invertase-AuNPs and BSA-AuNPs.

Procedure

Creating the Gold Stock Solution

  1. Add 0.0524 g of HAuCl4·3H2O to a 50 mL volumetric flask and fill with distilled water.
  2. The final concentration of gold stock was 2.66 mM.

Creating the Invertase Stock Solution

  1. Add 0.0717 g of lysozyme to a 50 mL volumetric flask and fill with distilled water.
  2. The final concentration of lysozyme stock was 100.28 μM.

Creating the BSA Stock Solution

  1. Add 0.0117 g of hemoglobin to a 50 mL volumetric flask and fill with distilled water.
  2. The final concentration of hemoglobin stock was 15 μM.


Solutions with the following Au:protein ratio were synthesized:

Gold to Hemoglobin Ratio
  • 30
  • 50
  • 70
  • 90
  • 110
  • 130
  • 150
  • 170
  • 190
  • 210
Gold to Lysozyme Ratio
  • 5
  • 10
  • 15
  • 20
  • 25
  • 30
  • 35
  • 40
  • 45
  • 50


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