Difference between revisions of "User:Allison K. Alix/Notebook/Thesis Research/2013/11/05"

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(To Do)
(Data)
Line 15: Line 15:
  
 
[[Image:OWW_AUNP_DNA_pic.png]]
 
[[Image:OWW_AUNP_DNA_pic.png]]
 +
 +
Calculating AuNP/DNA Concentration==
 +
 +
AuNP: abs @ 532 = 0.03
 +
ε = 92,382,878
 +
 +
c= A/εb
 +
c = 3.2473x10<sup>-10</sup>M
 +
  = 0.325nM
 +
 +
DNA: abs @ 254 = 0.399
 +
ε = 607,500
 +
c = 6.5679E-07
 +
  = 656.6nM
 +
  
  

Revision as of 09:49, 5 November 2013

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To Do

  • Run UV-Vis of MB samples to determine concentraiton
  • Add MB to AuNP/DNA Sample. Run FCS.

Data

OWW AUNP DNA pic.png

Calculating AuNP/DNA Concentration==

AuNP: abs @ 532 = 0.03 ε = 92,382,878

c= A/εb c = 3.2473x10-10M

 = 0.325nM

DNA: abs @ 254 = 0.399 ε = 607,500 c = 6.5679E-07

 = 656.6nM