User:Allison K. Alix/Notebook/CHEM-581/2013/03/08
Measuring Dye Absorbance | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Objectives-Suspend hydrogels back into solution -Attach dye to hydrogels once they are in solution -Measure the amount of dye absorbed Procedures1) Add 0.1 g of hydrogel (1 sample with lecithin and one without) in 5mL phosphate buffer 2) Sonicate for ~25 minutes or until hydrogels have separated 3) Add 5 μL of dye. 4) Allow hydrogels to absorb dye overnight 5) Calculate the amount of dye absorbed by the hydrogels by measuring the concentration left in solution Data
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