Difference between revisions of "Talk:Beta-Galactosidase Assay (A better Miller)"

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(New page: *'''~~~~''': It would be useful to know how long the permeabilization, substrate and stop solutions keep. Presumably, since the permeabilization and substrate solutions each contains &bet...)
 
 
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*'''[[User:Reshma P. Shetty|Reshma]] 18:55, 17 September 2007 (EDT)''': It would be useful to know how long the permeabilization, substrate and stop solutions keep.  Presumably, since the permeabilization and substrate solutions each contains β-mercaptoethanol, they need to be made fresh each time?
 
*'''[[User:Reshma P. Shetty|Reshma]] 18:55, 17 September 2007 (EDT)''': It would be useful to know how long the permeabilization, substrate and stop solutions keep.  Presumably, since the permeabilization and substrate solutions each contains β-mercaptoethanol, they need to be made fresh each time?
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**'''[[User:Reshma P. Shetty|Reshma]] 11:30, 15 October 2007 (CDT)''': The substrate solution is traditionally made fresh each day because solutions with ONPG only keep for a few days at 4°C.

Latest revision as of 08:30, 15 October 2007

  • Reshma 18:55, 17 September 2007 (EDT): It would be useful to know how long the permeabilization, substrate and stop solutions keep. Presumably, since the permeabilization and substrate solutions each contains β-mercaptoethanol, they need to be made fresh each time?
    • Reshma 11:30, 15 October 2007 (CDT): The substrate solution is traditionally made fresh each day because solutions with ONPG only keep for a few days at 4°C.