TBS
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Purpose
- TBS stands for tris-buffered saline and is used in many protocols for washing
Recipe
- Endconzentration in 1x TBS will be 20mM Tris and 150mM NaCl
for 1 liter with stock solutions |
---|
100 ml 1M Tris-HCl pH 7.5 |
150 ml 5M NaCl |
750 ml ddH2O |
- Prepare and sterile filter. Store at 4°C. After diluting the 5x to 1x filter again
Contact
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