Difference between revisions of "Sack and Yarov-Yarovoy:Protocols"

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(General Lab)
(Molecular Biology)
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==Molecular Biology==
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==molecular biology==
 
[[Sack: Bacteria Transformation (One Shot Top Ten Invitrogen)| Bacteria Transformation (One Shot Top Ten Invitrogen)]][http://openwetware.org/images/8/8e/Bacteria_Transformation_%28One_Shot_Top10_Invitrogen%29.pdf  .]<br>
 
[[Sack: Bacteria Transformation (One Shot Top Ten Invitrogen)| Bacteria Transformation (One Shot Top Ten Invitrogen)]][http://openwetware.org/images/8/8e/Bacteria_Transformation_%28One_Shot_Top10_Invitrogen%29.pdf  .]<br>
 
Bacteria Transformation Invitrogen  [http://openwetware.org/images/0/00/Bacteria_Transformation_Invitrogen.pdf  .]<br>
 
Bacteria Transformation Invitrogen  [http://openwetware.org/images/0/00/Bacteria_Transformation_Invitrogen.pdf  .]<br>
 
Growth of Bacterial Cultures [http://openwetware.org/images/3/39/QIAGEN_-_Growth_of_bacterial_cultures.pdf  .]<br>
 
Growth of Bacterial Cultures [http://openwetware.org/images/3/39/QIAGEN_-_Growth_of_bacterial_cultures.pdf  .]<br>
 
Subcloning Efficiency DH5a Chemically Competent E. Coli [http://openwetware.org/images/d/df/Subcloning_Efficiency_DH5a_Chemically_competent_E._Coli.pdf  .]<br>
 
Subcloning Efficiency DH5a Chemically Competent E. Coli [http://openwetware.org/images/d/df/Subcloning_Efficiency_DH5a_Chemically_competent_E._Coli.pdf  .]<br>
 
  
 
==Tentagel Azide Beads==
 
==Tentagel Azide Beads==

Revision as of 11:08, 25 October 2012

cell culture

Cell Media with Selection Agents (Blasticidin, Zeocin, Geneticin) .
Cell Media with Selection Agents (Blasticidin, Zeocin) .
Cell Splitting Protocol.
Cell Transfection (Lipofectamine LTX).
Cell Transfection (Lipofectamine).
Cell Transfection (Novachoice).
Cell Harvesting for Electrophysiology.
Cell Plating for Electrophysiology .
Freezing CHO Cells.
Plating Subclones for Ephys.
Plating Subclones for Fluorescent Screening.
Subcloning Cells.
Testing TREX Subclones Ephys.
Thawing CHO cells.

electrophysiology

Agar Bridge Formation.

general lab

Acidwash 03/22/11.

HPLC

BTAA Peptide Click HPLC 01/03/12.
Dylight Protocol 02/06/12.
Fmal Protocol.
HPLC Protocol.
Mass Spec Protocol for GxTx-13ACM.


Solutions

Ephys Solutions 08/15/12.
Example 20K External 11/08/11.
Reagent Stocks Template 11/21/11.
Solutions Template 11/22/11.


molecular biology

Bacteria Transformation (One Shot Top Ten Invitrogen).
Bacteria Transformation Invitrogen .
Growth of Bacterial Cultures .
Subcloning Efficiency DH5a Chemically Competent E. Coli .

Tentagel Azide Beads

Bead Cell Binding 05/15/12.
Beads Alykne Azide BTTAA 05/16/12.

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