Difference between revisions of "Maloof Lab:MS plates2"

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#Fill with Milli-Q water to exactly 1 liters
 
#Fill with Milli-Q water to exactly 1 liters
 
#Put 8 g (for vertical plate) or 7 g (for horizontal plate) of Agar in 1 clean glass bottle
 
#Put 8 g (for vertical plate) or 7 g (for horizontal plate) of Agar in 1 clean glass bottle
#Autoclave for 20 min
+
#Autoclave for 30 min
  
  

Latest revision as of 12:50, 11 December 2012

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Room 2115
Section of Plant Biology
1002 Life Sciences, One Shields Ave.
University of California Davis
Davis, CA 95616

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MS plates for plant growth (buffered, no sucrose)


This protocol will make 1 liters. Adjust to your needs if necessary.

  1. Final concentration of this medium is 1/2 MSMO, 5mM MES (pH5.8), 0.8% (or 0.7%) agar.
  2. Put 700 ml Milli-Q H2O in a beaker
  3. Weight 2.2 g of MSMO (in the 4°C freezer) and add it to the water (agitating)
  4. Add 1.0g of MES (2-(N-morpholino)ethanesulfonic acid)
  5. Adjust pH to 5.8 (adding KOH)
  6. Fill with Milli-Q water to exactly 1 liters
  7. Put 8 g (for vertical plate) or 7 g (for horizontal plate) of Agar in 1 clean glass bottle
  8. Autoclave for 30 min


Let cool, add antibiotic to your convenience and pour into plates
Big round plates take about 50 ml of media
Square plates take 25 ml of media (for horizontal plate) or 40 ml of media (for vertical plate).

created by Kazu (Oct 25, 2012)