Difference between revisions of "MOPS"

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[[Image:800px-Mops2.JPG|right|200px|thumb|The other mops]]
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'''MOPS''' is the common name for the buffering compound in MOPS buffer. MOPS stands for ''3-(N-morpholino) propanesulfonic acid'' and with a pKa of 7.20, MOPS is an good buffer for many biological systems at almost neutral pH. [[HEPES]] is a chemically similar pH buffering compound.  
 
'''MOPS''' is the common name for the buffering compound in MOPS buffer. MOPS stands for ''3-(N-morpholino) propanesulfonic acid'' and with a pKa of 7.20, MOPS is an good buffer for many biological systems at almost neutral pH. [[HEPES]] is a chemically similar pH buffering compound.  
  
 
==Recipe for 10x MOPS buffer==
 
==Recipe for 10x MOPS buffer==
  
[[Image:800px-MOPS.png|right|200px|chemical structure of MOPS, 3-(N-morpholino) propanesulfonic acid]]
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[[Image:800px-MOPS.png|right|200px|thumb|chemical structure of MOPS, 3-(N-morpholino) propanesulfonic acid]]
  
 
* 41.2g 3-(N-morpholino) propanesulfonic acid (MOPS)
 
* 41.2g 3-(N-morpholino) propanesulfonic acid (MOPS)

Revision as of 07:47, 2 March 2009

The other mops

MOPS is the common name for the buffering compound in MOPS buffer. MOPS stands for 3-(N-morpholino) propanesulfonic acid and with a pKa of 7.20, MOPS is an good buffer for many biological systems at almost neutral pH. HEPES is a chemically similar pH buffering compound.

Recipe for 10x MOPS buffer

chemical structure of MOPS, 3-(N-morpholino) propanesulfonic acid
  • 41.2g 3-(N-morpholino) propanesulfonic acid (MOPS)
  • 10.9g Sodium Acetate, 3- hydrate
  • 3.7g EDTA, sodium salt


  • add 800ml of nuclease free distilled water; mix to dissolve
  • adjust to pH 7 with NaOH (prepared in nuclease free distilled water)
  • fill to the final volume of 1000 ml


  • filter sterilise
  • store at room temperature
  • protect from light; do not use if the solution appears yellow

Some OWW protocols which use MOPS

External links