Difference between revisions of "IGEM:Peking University/2008/Experiment/PKU 080512 G1 1"

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(New page: ==PKU_080512_G1_1== ::'''Transformation-pGREG505/TEF-lacI-adhlacS-GFP-E.Coli-Amp+,URA''' {|align="right" 200px |} {|border="1" cellspacing="0" |+ |- | ||Volume (μL) ...)
 
 
(10 intermediate revisions by one other user not shown)
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==PKU_080512_G1_1==
 
==PKU_080512_G1_1==
 +
<hr class=divider>
 
::'''Transformation-pGREG505/TEF-lacI-adhlacS-GFP-E.Coli-Amp+,URA'''
 
::'''Transformation-pGREG505/TEF-lacI-adhlacS-GFP-E.Coli-Amp+,URA'''
 
{|align="right"
 
{|align="right"
[[PKU_photo1.jpg|200px]]
+
[[Image:PKU_080512_ADH-plasmid-check(95min).tif|400px]]
 
|}
 
|}
  
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<hr class=divider>
  
 
::'''PCR- pGREG505/(TEF-lacI-adhlacS-GFP)-adhlac-50ul'''
 
::'''PCR- pGREG505/(TEF-lacI-adhlacS-GFP)-adhlac-50ul'''
 
{|align="right"
 
{|align="right"
[[PKU_20080512_adhpcrcheck.tif|200px]]
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[[Image:PKU_080512_ADH-PCR-check.tif|200px]]
 
|}
 
|}
 
{|border="1" cellspacing="0"  
 
{|border="1" cellspacing="0"  
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|+  
 
|+  
 
|-
 
|-
|Temperature(&&&)||Time Length(0:00:00)
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|Temperature(°C)||Time Length(0:00:00)
 
|-  
 
|-  
 
| 94||05:00
 
| 94||05:00
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| Alarm||5 times
 
| Alarm||5 times
 
|-
 
|-
| Hold||4&&&
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| Hold||4°C
 +
|}
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 +
{|align="right"
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|back to [[PKU_Experiment|Previous Page]]
 
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Latest revision as of 21:46, 11 July 2008

PKU_080512_G1_1


Transformation-pGREG505/TEF-lacI-adhlacS-GFP-E.Coli-Amp+,URA
File:PKU 080512 ADH-plasmid-check(95min).tif
Volume (μL)
DNA 5
ClaI 0.2
BamH1 0.2
KBuffer 2
ddH2O 12.6



PCR- pGREG505/(TEF-lacI-adhlacS-GFP)-adhlac-50ul
File:PKU 080512 ADH-PCR-check.tif
Volume (μL)
pGREG505/(TEF-lacI-adhlacS-GFP) 5
dNTP 5
KOD Plus 1
KOD Plus Buffer 5
MgSO4 2
F-Primer 1
R-Primer 1
ddH2O 34


conditions
Temperature(°C) Time Length(0:00:00)
94 05:00
94 00:30 30 cycles
55 00:30 30 cycles
72 01:30 30 cycles
72 07:00
Alarm 5 times
Hold 4°C
back to Previous Page