IGEM:Peking University/2008/Experiment/PKU 080512 G1 1
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PKU_080512_G1_1
- Transformation-pGREG505/TEF-lacI-adhlacS-GFP-E.Coli-Amp+,URA
| Volume (μL) | |
| DNA | 5 |
| ClaI | 0.2 |
| BamH1 | 0.2 |
| KBuffer | 2 |
| ddH2O | 12.6 |
- PCR- pGREG505/(TEF-lacI-adhlacS-GFP)-adhlac-50ul
| Volume (μL) | |
| pGREG505/(TEF-lacI-adhlacS-GFP) | 5 |
| dNTP | 5 |
| KOD Plus | 1 |
| KOD Plus Buffer | 5 |
| MgSO4 | 2 |
| F-Primer | 1 |
| R-Primer | 1 |
| ddH2O | 34 |
- conditions
| Temperature(°C) | Time Length(0:00:00) | |
| 94 | 05:00 | |
| 94 | 00:30 | 30 cycles |
| 55 | 00:30 | 30 cycles |
| 72 | 01:30 | 30 cycles |
| 72 | 07:00 | |
| Alarm | 5 times | |
| Hold | 4°C |
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