IGEM:Peking University/2008/Experiment/PKU 080512 G1 1
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PKU_080512_G1_1
- Transformation-pGREG505/TEF-lacI-adhlacS-GFP-E.Coli-Amp+,URA
![](https://s3-us-west-2.amazonaws.com/oww-files-thumb/0/0a/PKU_080512_ADH-plasmid-check%2895min%29.tif/271px-PKU_080512_ADH-plasmid-check%2895min%29.tif.png)
Volume (μL) | |
DNA | 5 |
ClaI | 0.2 |
BamH1 | 0.2 |
KBuffer | 2 |
ddH2O | 12.6 |
- PCR- pGREG505/(TEF-lacI-adhlacS-GFP)-adhlac-50ul
![](https://s3-us-west-2.amazonaws.com/oww-files-thumb/1/1d/PKU_080512_ADH-PCR-check.tif/128px-PKU_080512_ADH-PCR-check.tif.png)
Volume (μL) | |
pGREG505/(TEF-lacI-adhlacS-GFP) | 5 |
dNTP | 5 |
KOD Plus | 1 |
KOD Plus Buffer | 5 |
MgSO4 | 2 |
F-Primer | 1 |
R-Primer | 1 |
ddH2O | 34 |
- conditions
Temperature(°C) | Time Length(0:00:00) | |
94 | 05:00 | |
94 | 00:30 | 30 cycles |
55 | 00:30 | 30 cycles |
72 | 01:30 | 30 cycles |
72 | 07:00 | |
Alarm | 5 times | |
Hold | 4°C |
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