IGEM:IMPERIAL/2006/project/Oscillator/project browser/Test Sensing Predator Construct/Implementation: Difference between revisions

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=Predator Test Construct=
<u><font size="2">'''Ligating the Polycystronic Predator 'Sensing' Test Construct:'''</font></u>
These two parts are very similar and have the same inputs and outputs, therefore they can be analysed in exactly the same way
__NOTOC__
{| border="1" cellpadding="0" width="100%"
|-
!style="background:#E6B3E0" height="25pt" width="80pt" |For !! style="background:#E6B3E0" |Stage 1 !! style="background:#E6B3E0" |Stage 2 !! style="background:#E6B3E0" |Stage 3
|-
!style="background:#D9F2ED"|<center><bbpart>J37033</bbpart>=<br>(<bbpart>B0034</bbpart>+<bbpart>C0062</bbpart>)</center> !!style="background:#CCEBAD" | <center>[[Image:B0034 logo.png]][[Image:Plus sign.png|20px]][[Image:C0062 logo.png]]</center>
|-
!style="background:#D9F2ED"|<center><bbpart>J37019</bbpart>=<br>(<bbpart>R0062</bbpart>+<bbpart>J37033</bbpart>)</center>|| !!style="background:#CCEBAD" | <center>[[Image:R0062 logo.png]][[Image:Plus sign.png|20px]][[Image:B0034 logo.png]][[Image:C0062 logo.png]]</center>
|-
!style="background:#D9F2ED"|<center><bbpart>J37016</bbpart>=<br>(<bbpart>J37019</bbpart>+<bbpart>J37025</bbpart>)</center>|| || !!style="background:#CCEBAD" | <center>[[Image:J37016 logo.png]]</center>
|}


<br>
<u><font size="2">'''Ligating the Monocystronic Predator 'Sensing' Test Construct:'''</font></u>
*<big>'''[http://parts.mit.edu/registry/index.php/Part:BBa_J37016 J37016]'''</big>
__NOTOC__
<br>
{| border="1" cellpadding="0" width="100%"
[[image:J37016.JPG]]
|-
 
!style="background:#E6B3E0" height="25pt" width="80pt" |For !! style="background:#E6B3E0" |Stage 1 !! style="background:#E6B3E0" |Stage 2 !! style="background:#E6B3E0" |Stage 3 !! style="background:#E6B3E0" |Stage 4
This construct is used to characterise the pLuxR transfer function, and noteably its effect on YFP expression, which would be equivalent to AiiA expression in the full system with one promoter.
|-
<br>
!style="background:#D9F2ED"|<center><bbpart>J37033</bbpart>=<br>(<bbpart>B0034</bbpart>+<bbpart>C0062</bbpart>)</center> !! style="background:#CCEBAD"| <center>[[Image:B0034 logo.png]][[Image:Plus sign.png|20px]][[Image:C0062 logo.png]]</center>
<br>
|-
*[http://parts.mit.edu/registry/index.php/Part:BBa_R0062 R0062] - 9G Plate 1
!style="background:#D9F2ED"|<center><bbpart>J37019</bbpart>=<br>(<bbpart>R0062</bbpart>+<bbpart>J37033</bbpart>)</center>|| !!style="background:#CCEBAD" |<center>[[Image:R0062 logo.png]][[Image:Plus sign.png|20px]][[Image:B0034 logo.png]][[Image:C0062 logo.png]]</center>
*[http://parts.mit.edu/registry/index.php/Part:BBa_B0034 B0034] - 3O Plate 1
|-
*[http://parts.mit.edu/registry/index.php/Part:BBa_C0062 C0062] - 7A Plate 1
!style="background:#D9F2ED"|<center><bbpart>J37031</bbpart>=<br>(<bbpart>J37019</bbpart>+<bbpart>B0014</bbpart>)</center>|| || !!style="background:#CCEBAD" | [[Image:J37031 logo.png]]
*[http://parts.mit.edu/registry/index.php/Part:BBa_I13504 I13504] - 12D Plate 1
|-
 
!style="background:#D9F2ED"|<center><bbpart>J37032</bbpart>=<br>(<bbpart>R0062</bbpart>+<bbpart>I13504</bbpart>)</center> !!style="background:#CCEBAD" | [[Image:R0062 logo.png]][[Image:Plus sign.png|20px]][[Image:I13504 logo.png]]
 
|-
<br>
!style="background:#D9F2ED"|<center><bbpart>J37020</bbpart>=<br>(<bbpart>J37031</bbpart>+<bbpart>J37032</bbpart>)</center>|| || || !!style="background:#CCEBAD" | [[Image:J37020 logo.png]]
*<big>'''[http://parts.mit.edu/registry/index.php/Part:BBa_J37020  J37020]'''</big>
|}
<br>
[[image:J37020.JPG]]
 
This construct is used to characterise the pLuxR transfer function, and noteably its effect on YFP expression, which would be equivalent to AiiA expression in the full system with two promoters.
 
The input is HSL and the outputs are LuxR and GFP. GFP will be mesured
 
*[http://parts.mit.edu/registry/index.php/Part:J37018 J37018] - To be built!
*[http://parts.mit.edu/registry/index.php/Part:I13504 I13504] - 12D Plate 1


==Quality control==
==Quality control==

Revision as of 10:16, 29 October 2006

Super Parts Predator Construct
Actual Part
Sub Parts <bbpart>R0062</bbpart> <bbpart>B0034</bbpart> <bbpart>C0062</bbpart> <bbpart>I13504</bbpart>


Ligating the Polycystronic Predator 'Sensing' Test Construct:

For Stage 1 Stage 2 Stage 3
<bbpart>J37033</bbpart>=
(<bbpart>B0034</bbpart>+<bbpart>C0062</bbpart>)
<bbpart>J37019</bbpart>=
(<bbpart>R0062</bbpart>+<bbpart>J37033</bbpart>)
<bbpart>J37016</bbpart>=
(<bbpart>J37019</bbpart>+<bbpart>J37025</bbpart>)

Ligating the Monocystronic Predator 'Sensing' Test Construct:

For Stage 1 Stage 2 Stage 3 Stage 4
<bbpart>J37033</bbpart>=
(<bbpart>B0034</bbpart>+<bbpart>C0062</bbpart>)
<bbpart>J37019</bbpart>=
(<bbpart>R0062</bbpart>+<bbpart>J37033</bbpart>)
<bbpart>J37031</bbpart>=
(<bbpart>J37019</bbpart>+<bbpart>B0014</bbpart>)
<bbpart>J37032</bbpart>=
(<bbpart>R0062</bbpart>+<bbpart>I13504</bbpart>)
<bbpart>J37020</bbpart>=
(<bbpart>J37031</bbpart>+<bbpart>J37032</bbpart>)

Quality control

  • These parts were sequenced to determine the correct sequence


Part Logo Built Sequencing Results Primers Used for Sequencing
<bbpart>BBa_J37016</bbpart> jkhkh Yes sequencing analysis and link to sequence results PSBA2 primers
<bbpart>BBa_J37020</bbpart> kljkljkl Yes sequencing analysis and link to sequence results PSBA2 primers
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