Haynes Lab:Notebook/Lab Traning - Pradyumna Kadambi/2013/06/28: Difference between revisions
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(Autocreate 2013/06/28 Entry for Haynes_Lab:Notebook/Lab_Traning_-_Pradyumna_Kadambi) |
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== | ==DH5α Incubation in Liquid Media 6/28/2013== | ||
#With a pipetter, 3mL of LB+amp was added to a test tube. | |||
#A colony was selected on the culture plate(plate was overgrown), and a circle was drawn where the colony was selected. | |||
#Micropipette tip was lightly touched to the colony making sure not to scrape any of the agar gel. Micropipette tip was then ejected into test tube. | |||
#Test tube was incubated with shaker for 7 hours at 37 degrees. | |||
==DH5α Mini Prep with KAH013 6/28/2013== | |||
*Colonies that Rene had incubated in liquid media on 6/27 were used. | |||
#Using a micropipette, a 2mL centrifuge tube was filled with the incubated liquid media. | |||
#The solution was centrifuged at 13.1g for 3 minutes (If a pellet has not completely condensed centrifuge again until pellet does condense). | |||
#Using a micropipette, the supernatant was removed until 600μL of solution remained in the centrifuge tube. (Discard supernatant into the bleach container in the fume hood) | |||
#Pellet was broken up using the vortex. | |||
#Added 100μL of lysis buffer to the centrifuge tube and inverted 4-6 times and waited 1 min. | |||
#Added 350μL of lysis buffer. Invert the solution until it turns yellow, make sure no blue pockets are left. | |||
#Centrifuge the solution at | |||
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Revision as of 20:53, 30 June 2013
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DH5α Incubation in Liquid Media 6/28/2013
DH5α Mini Prep with KAH013 6/28/2013
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