Haynes Lab:Notebook/Lab Traning - Pradyumna Kadambi/2013/06/25

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DH5α Transformation with KAH013 Plasmid 6/25/2013

  1. In ice bath, 1μl KAH013 plasmid and 50μl competent E.Coli were combined.
  2. Waited 10 minutes for bacteria to accept plasmid.
  3. Transformed bacteria were then added to Agarose gel petri dish, rolling beads were used to evenly spread bacteria.
  4. Petri dish was labeled with initials date and plasmid name("PK 6/25/2013 KAH13") and was placed in incubator at 37 degrees for two days(6/25-6/26).
  5. Resultant colonies were picked by Rene and grown in liquid LB media overnight(6/27/2013).