Dahlquist:Protocols: Difference between revisions
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(→Wet Lab Protocols: changed BioRad lit name) |
(→Wet Lab Protocols: separated out Real-time PCR, ABI guide) |
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* [[Media:DahlquistLab_CompetentCellPreparation.doc | E. coli Chemically Competent Cell Preparation Protocol]] | * [[Media:DahlquistLab_CompetentCellPreparation.doc | E. coli Chemically Competent Cell Preparation Protocol]] | ||
** [[Dahlquist:Transformation Protocol for E. coli Chemically Competent Cells]] | ** [[Dahlquist:Transformation Protocol for E. coli Chemically Competent Cells]] | ||
* [[Dahlquist:Kodak Gel Logic 100 | Kodak Gel Logic 100]] | |||
=== Real-time PCR === | |||
* [http://www.bio-rad.com//webroot/web/pdf/lsr/literature/PN_10010421.pdf Bio-Rad CFX Manager Plate Quick Guide] | * [http://www.bio-rad.com//webroot/web/pdf/lsr/literature/PN_10010421.pdf Bio-Rad CFX Manager Plate Quick Guide] | ||
* [ | * [http://www3.appliedbiosystems.com/cms/groups/mcb_support/documents/generaldocuments/cms_039283.pdf ABI TaqMan Gene Expression Master Mix Quick Reference Card] | ||
== Dry Lab Protocols == | == Dry Lab Protocols == | ||
Revision as of 10:15, 1 October 2009
Lab Organization
- Laboratory Safety and Citizenship Rules
- Student Contact Information Form
- Guidelines for Paper Laboratory Notebooks
- Check-out Procedure for leaving the lab
- Temperature Log Template (for recording daily temperatures of freezers, incubators, etc.)
Wet Lab Protocols
- DNA Microarray Protocol: this protocol is under construction.
- Testing Yeast Strains for Temperature-sensitive Growth on Solid Media
- YEPD Medium
- YEPD Plates
- Autoclave instructions
- Yeast Colony PCR
- For genotyping yeast systematic deletion strains as adapted for the Dahlquist Lab
- Scanning instructions GenePix 4000B
- E. coli Chemically Competent Cell Preparation Protocol
- Kodak Gel Logic 100
Real-time PCR
Dry Lab Protocols
- Edge Software Protocol
- Edge Manual (Note: I posted this because I could no longer find a link to it on the web.)
- Dahlquist:Wiki Checklist
- Changing Drive Letters on Windows XP
