Buckner Lab:Notebook/Summer 2010/2010/05/19

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Plasmid Isolation

  • Used QIAprep isolation kit from Qiagen to isolate plasmids.
  • Performed EcoR1 digestion to isolated DNA for ~2.25 hrs

Pouring and Running Gel

  • Made a 0.8% agarose gel with ethidium bromide
  • Ran the gel for 1.5 hrs in TAE buffer at 60V.
  • Photographed and annotated gels

-Didn't allow the gel to run long enough.
-Need to add all of the DNA from the prep tube when taking a "look" after an EcoRI digestion

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