Plasmid Isolation and Liquid Media
Objectives:
- Analyze bacterial cultures inoculated 5/17
- Prepare LB Broth medium for the Synthetic Biology Lab
- Make a ampicillin stock for the SynBio Lab.
- Make overnight cultures
Bacteria Results
fas1b 2, KER 1D, Kn1 Recombined Plasmids in E.coli JM109
Bacterial Strain |
Growth (+/-) |
Description
|
JM 109 25μL
|
+ |
Restreak (MC)
|
JM 109 100μL
|
+
|
Good Growth (MC)
|
Kn1 25μL
|
+ |
Sm iso colonies
|
Kn1 100μL
|
+ |
MC to isolate
|
KER 1D 25μL
|
+ |
Sm iso colonies
|
KER 1D 100μL
|
+ |
MC to isolate
|
fas1b 2 25μL
|
+ |
iso colonies
|
fas1b 2 100μL
|
+ |
MC to isolate
|
MC= "multiple colonies present on agar"
LB Broth and Ampicillin Stock
-Made 300mL of LB broth media. Autoclaved at 9:47am
-Poured broth into 6, 50mL portions. Stored in SynBio Lab at room temp
-Started to make 10mL Amp (50mg/mL concentration) but spilled some during sterilization. Ended making 6mL of Amp stock and separating into 7 x's 700μL portions. Placed sterilized Amp in bullet box in Buckner freezer.
Back to Buckner Lab
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