BE.109:Bio-material engineering: Difference between revisions
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==Module 4== | ==Module 4== | ||
In this experimental module you will study an unusual protein, one that allows yeast to bind a metal, gold. Over the next few weeks you will purify yeast based on this binding property, and then you’ll vary some experimental condition to improve the yeast/gold interaction. Using your optimized conditions, you will screen a library of yeast to isolate a new gold-binding strain. The DNA encoding your new gold-binding protein will be sequenced and, with any luck, you’ll elucidate some amino acid requirements for the yeast/metal interaction. | In this experimental module you will study an unusual protein, one that allows yeast to bind a metal, gold. Over the next few weeks you will purify yeast based on this binding property, and then you’ll vary some experimental condition to improve the yeast/gold interaction. Using your optimized conditions, you will screen a library of yeast to isolate a new gold-binding strain. The DNA encoding your new gold-binding protein will be sequenced and, with any luck, you’ll elucidate some amino acid requirements for the yeast/metal interaction. | ||
==Lab handouts== | |||
'''[[BE.109:Bio-material engineering day 1 | Day 1]]''' | |||
'''[[BE.109:Bio-material engineering day 2 | Day 2]]''' | |||
'''[[BE.109:Bio-material engineering day 3 | Day 3]]''' | |||
'''[[BE.109:Bio-material engineering day 4 | Day 4]]''' | |||
'''[[BE.109:Bio-material engineering day 5 | Day 5]]''' | |||
Revision as of 15:50, 20 November 2005
Module 4
In this experimental module you will study an unusual protein, one that allows yeast to bind a metal, gold. Over the next few weeks you will purify yeast based on this binding property, and then you’ll vary some experimental condition to improve the yeast/gold interaction. Using your optimized conditions, you will screen a library of yeast to isolate a new gold-binding strain. The DNA encoding your new gold-binding protein will be sequenced and, with any luck, you’ll elucidate some amino acid requirements for the yeast/metal interaction.
