Difference between revisions of "Altman:Protocols/Preparing Reagents/Making GB"

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(Making Glucose Beta-Mercaptoethanol (G/β))
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FINAL CONCENTRATION of 50x G/β stock:
 
FINAL CONCENTRATION of 50x G/β stock:
 
+
* 22.5% (w/v) Glucose
22.5% (w/v) Glucose
+
* 12.5% (v/v)  β-mercaptoethanol  
 
 
12.5% (v/v)  β-mercaptoethanol  
 
 
 
in assay buffer
 
  
 
Final concentration in experiment: 0.45 % (w/v) Glucose, 0.25% (v/v) β-mercaptoethanol
 
Final concentration in experiment: 0.45 % (w/v) Glucose, 0.25% (v/v) β-mercaptoethanol

Revision as of 13:24, 17 November 2012

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Making Glucose Beta-Mercaptoethanol (G/β)

FINAL CONCENTRATION of 50x G/β stock:

  • 22.5% (w/v) Glucose
  • 12.5% (v/v) β-mercaptoethanol

Final concentration in experiment: 0.45 % (w/v) Glucose, 0.25% (v/v) β-mercaptoethanol

1 mL G/β Glucose 0.225 g 14.3 M β-mercaptoethanol 125 μL

1x AB 875 μL