840:153g:Projects/project23/2012/11/08

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Transforming E. coli with omcf gene

  • On Tuesday, we extracted our DNA from gels stored 11.02.12. We used a gel-extraction kit and extracted both the 350- and 450-bp regions in 100 mcl of water each.
  • On Thursday, we digested the DNA samples from 11.08.12 using restriction enzymes PstI and XbaI for our Biobrick assembly. We then purified the reaction with a purification kit to remove restriction enzymes and stored the product at -20°C for further ligation next week.
  • On Thursday, we also prepared and shipped DNA samples from our 350- and 450-bp target regions from Tuesday for sequencing. We should get the results next week.