840:153g:Projects/project23/2012/11/08

Project name

Transforming E. coli with omcf gene

On Tuesday, we extracted our DNA from gels stored 11.02.12. We used a gel-extraction kit and extracted both the 350- and 450-bp regions in 100 mcl of water each.
On Thursday, we digested the DNA samples from 11.08.12 using restriction enzymes PstI and XbaI for our Biobrick assembly. We then purified the reaction with a purification kit to remove restriction enzymes and stored the product at -20°C for further ligation next week.
On Thursday, we also prepared and shipped DNA samples from our 350- and 450-bp target regions from Tuesday for sequencing. We should get the results next week.