840:153g:Projects/project23/2012/10/26: Difference between revisions
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==Site-directed Mutagenesis== | ==Site-directed Mutagenesis== | ||
* On | *On Friday, we ran Thursday's PCR products on a 1.8% Agarose gel. The samples were loaded as follows: | ||
* | *Upper lane | ||
# | #100bp ladder | ||
# | #Non-primered set without template | ||
#Upstream fragment without template | |||
#Primered set without template | |||
# | #Downstream fragment without template | ||
# | |||
# | |||
#100 bp ladder | #100 bp ladder | ||
#Downstream fragment | |||
#Primered set | |||
#Upstream fragment | |||
#Non-primered set | |||
* | *Lower lane | ||
#Positive control | |||
#Positive | |||
#100 bp ladder | #100 bp ladder | ||
*All other lanes are replications of primered set | |||
* | *Results: | ||
*Upper lane, wells 1-5 | |||
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__NOTOC__ | __NOTOC__ | ||
[[category:OWWLabNotebookV1]] | [[category:OWWLabNotebookV1]] |
Revision as of 16:26, 29 October 2012
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Site-directed Mutagenesis
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