840:153g:Projects/project23/2012/11/01

Cloning of the OmcF gene

Continuation of Mutagenesis Reaction

On Tuesday, repeated the PCR preparation that we completed last Thursday. The reason for repeating this experiment was to be able to cut out the bands that contained our supposed gene. Another reason is to be able to clearly compare the bands side by side, which wasn't clear the first time we preformed the PCR and gel electrophoresis.
On Thursday we prepared two gels, one at 1.0% and the other at 1.8%. We ran the only the replicated primered DNA samples in the 1.0% gel for further extraction. Everything else was run on the 1.8% gel for higher resolution. We ran them overnight at 10V in order to create clearer bands.