Difference between revisions of "3-Aminotriazole"

From OpenWetWare
Jump to: navigation, search
m
 
(One intermediate revision by the same user not shown)
Line 1: Line 1:
3-amino-1,2,4-triazole (also called 3-AT) is a competitive inhibitor of the HIS3 gene product (imidazoleglycerol-phosphate dehydratase). Adding 3-AT to the media raises the stringency of the HIS3 selection. Frequently used in two-hybrid screens to eliminate background colonies.
+
==Purpose==
 +
*3-amino-1,2,4-triazole (also called 3-AT) is a competitive inhibitor of the HIS3 gene product (imidazoleglycerol-phosphate dehydratase). Adding 3-AT to the media raises the stringency of the HIS3 selection. Frequently used in two-hybrid screens to eliminate background colonies.
  
Stored as a powder at -20. Working concentrations vary depending on the desired stringency, from 0.2 mM to 25mM.
+
==Procurement==
 +
*You can buy 3-amino-1,2,4-triazole for example at [http://www.sigmaaldrich.com Sigma].
  
Possible carcinogen. Use gloves, work in a hood.
+
==Use==
 +
*Stored as a powder at -20. Working concentrations vary depending on the desired stringency, from 0.2 mM to 25mM.
  
References:
+
==Safety==
[http://www.sigmaaldrich.com/sigma/product%20information%20sheet/a8056pis.pdf Sigma product information]
+
*Possible carcinogen. Use gloves, work in a hood.
 +
 
 +
==References==
 +
*[http://www.sigmaaldrich.com/sigma/product%20information%20sheet/a8056pis.pdf Sigma product information]
  
 
[[Category:Material]] [[Category:Chemical]]
 
[[Category:Material]] [[Category:Chemical]]

Latest revision as of 00:54, 7 March 2008

Purpose

  • 3-amino-1,2,4-triazole (also called 3-AT) is a competitive inhibitor of the HIS3 gene product (imidazoleglycerol-phosphate dehydratase). Adding 3-AT to the media raises the stringency of the HIS3 selection. Frequently used in two-hybrid screens to eliminate background colonies.

Procurement

  • You can buy 3-amino-1,2,4-triazole for example at Sigma.

Use

  • Stored as a powder at -20. Working concentrations vary depending on the desired stringency, from 0.2 mM to 25mM.

Safety

  • Possible carcinogen. Use gloves, work in a hood.

References