Lidstrom:Sorvall Centrifuge
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Picture
![](https://s3-us-west-2.amazonaws.com/oww-files-thumb/1/1f/Lidstrom_Sorvoll.png/300px-Lidstrom_Sorvoll.png.jpeg)
Rotors
Rotors we have:
- SS-34. Holds 8*50 mL, spins at 20,000 RPM/36,900 RCF
- GS-3.
- For bigger bottles.
- ____. Holds eppendorf (1.5mL) tubes
- For reference, the benchtop 50mL centrifuges (e.g. ST-15R with a TX-400 swinging bucket rotor) we have spin at 4696 RCF.
- There are stickers with maximum speeds. These were set by Katya, a Lidstrom Lab manager, circa 2011. It is unknown what the motivation was for keeping the rotors below maximum speed.
![](https://s3-us-west-2.amazonaws.com/oww-files-thumb/8/8c/Lidstrom_sorvoll_speed_instructions.png/600px-Lidstrom_sorvoll_speed_instructions.png.jpeg)
![](https://s3-us-west-2.amazonaws.com/oww-files-thumb/8/8b/SS-34_rotor_speeds_and_k_factors.png/600px-SS-34_rotor_speeds_and_k_factors.png)
![](https://s3-us-west-2.amazonaws.com/oww-files-thumb/d/de/GS-3_rotor_speeds_and_k_factors.png/600px-GS-3_rotor_speeds_and_k_factors.png)
![](https://s3-us-west-2.amazonaws.com/oww-files-public/3/3a/RCF_explanation.png)
Instructions
Example protocol for AM1:
Janet's SIP3-4 centrifugation:
- Chill to 4oC with SS-34 rotor inside
- Fill correct tubes
- Spin for ___ min at ___ RCF at ___oC