Gene Target |
Forward Primer Name |
Forward sequence |
Reverse Primer Name |
Reverse sequence |
product size |
purpose |
remarks
|
GFP
|
GFP-T7-Forward
|
TAATACGACTCACTATAGGG CAAGACACGTGCTGAAGTCAA
|
GFP-T7-Reverse
|
TAATACGACTCACTATAGGG GCCTGAATTTAACCAGGAACC
|
~450bp
|
w/ T7 overhang, for RNAi
|
Smeister
|
LacZ
|
LacZ-T7-Forward
|
TAATACGACTCACTATAGGG AGAATCCGACGGGTTGTTACT
|
LacZ-T7-Reverse
|
TAATACGACTCACTATAGGG CACCACGCTCATCGATAATTT
|
~550bp
|
w/ T7 overhang, for RNAi
|
Smeister
|
ribosomal S7
|
S7-A
|
GGCGATCATCATCTACGTGC
|
S7-B
|
GTAGCTGCTGCAAACTTCGG
|
460bp
|
semiquantitative RT-PCR
|
Smeister
|
ribosomal S7
|
AgS7 qRT-PCR F
|
GTGCGCGAGTTGGAGAAGA
|
AgS7 qRT-PCR R
|
ATCGGTTTGGGCAGAATGC
|
78bp
|
quantitative RT-PCR
|
Smeister
|
|
M13/pUC-Forward
|
CGCCAGGGTTTTCCCAGTCACGAC
|
M13/pUC-Reverse
|
AGCGGATAACAATTTCACACAGGA
|
|
plasmid sequencing
|
Stephan
|
|
SP6 Promoter
|
CATACGATTTAGGTGACACTATAG
|
|
|
|
plasmid sequencing
|
Smeister
|
|
T7 Promoter 20mer
|
TAATACGACTCACTATAGGG
|
T3 Promoter 23mer
|
GCAATTAACCCTCACTAAAGGGA
|
|
plasmid sequencing
|
Smeister
|
|
T7-d(T)24
|
GGCCAGTGAATTGTAATACGACTCACTATAGGGAGGCGG(T)24
|
|
|
|
microarray
|
Smeister
|
|