Kafatos:Primers

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Gene Target Forward Primer Name Forward sequence Reverse Primer Name Reverse sequence product size purpose remarks GFP GFP-T7-Forward TAATACGACTCACTATAGGG CAAGACACGTGCTGAAGTCAA GFP-T7-Reverse TAATACGACTCACTATAGGG GCCTGAATTTAACCAGGAACC ~450bp w/ T7 overhang, for RNAi Smeister LacZ LacZ-T7-Forward TAATACGACTCACTATAGGG AGAATCCGACGGGTTGTTACT LacZ-T7-Reverse TAATACGACTCACTATAGGG CACCACGCTCATCGATAATTT ~550bp w/ T7 overhang, for RNAi Smeister ribosomal S7 S7-A GGCGATCATCATCTACGTGC S7-B GTAGCTGCTGCAAACTTCGG 460bp semiquantitative RT-PCR Smeister ribosomal S7 AgS7 qRT-PCR F GTGCGCGAGTTGGAGAAGA AgS7 qRT-PCR R ATCGGTTTGGGCAGAATGC 78bp quantitative RT-PCR Smeister M13/pUC-Forward CGCCAGGGTTTTCCCAGTCACGAC M13/pUC-Reverse AGCGGATAACAATTTCACACAGGA plasmid sequencing Stephan SP6 Promoter CATACGATTTAGGTGACACTATAG plasmid sequencing Smeister T7 Promoter 20mer TAATACGACTCACTATAGGG T3 Promoter 23mer GCAATTAACCCTCACTAAAGGGA plasmid sequencing Smeister T7-d(T)24 GGCCAGTGAATTGTAATACGACTCACTATAGGGAGGCGG(T)24 microarray Smeister